29-173
Aislamiento y cultivo de células osteoblásticas: Interés para la investigación en cirugía ortopédica y traumatología
ISOLATION AND CHARACTERIZATION OF OSTEOBLASTIC CELLS: ITS VALUE IN ORTHOPAEDIC RESEARCH
L. MESEGUER OLMO, A. BERNABEU ESCLAPEZ, M. CLAVEL-SAINZ NOLLA, J. MUÑOZ RUIZ-SEIQUER y L. RUANO GARCÍA Servicio de COT, Hospital Universitario «Morales Meseguer», Murcia. Unidad de Cultivos Celulares, Universidad de Murcia. Servicio de COT, Hospital Universitario «Virgen de la Arrixaca», Murcia. Laboratorio de Cirugía Ortopédica Experimental (Prof. Clavel-Sainz). Facultad de Medicina, Murcia.
Recepción:
09/01/2009
Aceptación:
09/01/2009
Publicación:
09/01/2009
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Resumen (ES)
We describe a method of isolation and characterization of osteoblastic cells taken
from the surface of bone trabeculae of explants obtained from femur and tibia metaphysis
in rabbits and human femoral head in patients undergoing orthopaedic surgery. Afther removing
the cells from the bone marrow of explants, they were placed over two mesh, 80 and
100 μmn, obtaining then a culture in monolayer which showed to be confluent after 20 days.
The isolated cell population showed an osteoblastic phenotype using colourmetric methods to
determinate the alkaline phosphatase (AF) activity and morphologic methods by means of
scanning. The cultivated cell showed a different morphology according to the degree of confluence
of the culture, being always positive the sucesive colourmetric determinations of AF
along the experiment. We consider the method of a great interest for orthopaedic investigations,
since it allows the study of the behaviour of these cells when they are subjet to different
types of external stimulus as well as meet they the new generations of biomaterials.
from the surface of bone trabeculae of explants obtained from femur and tibia metaphysis
in rabbits and human femoral head in patients undergoing orthopaedic surgery. Afther removing
the cells from the bone marrow of explants, they were placed over two mesh, 80 and
100 μmn, obtaining then a culture in monolayer which showed to be confluent after 20 days.
The isolated cell population showed an osteoblastic phenotype using colourmetric methods to
determinate the alkaline phosphatase (AF) activity and morphologic methods by means of
scanning. The cultivated cell showed a different morphology according to the degree of confluence
of the culture, being always positive the sucesive colourmetric determinations of AF
along the experiment. We consider the method of a great interest for orthopaedic investigations,
since it allows the study of the behaviour of these cells when they are subjet to different
types of external stimulus as well as meet they the new generations of biomaterials.
Palabras clave (ES):
Cultivo
Investigación
celular
Células
oseoblásticas
Osteoblasto
Preosteoblasto
Indiferenciada
Resumen (EN)
We describe a method of isolation and characterization of osteoblastic cells taken
from the surface of bone trabeculae of explants obtained from femur and tibia metaphysis
in rabbits and human femoral head in patients undergoing orthopaedic surgery. Afther removing
the cells from the bone marrow of explants, they were placed over two mesh, 80 and
100 μmn, obtaining then a culture in monolayer which showed to be confluent after 20 days.
The isolated cell population showed an osteoblastic phenotype using colourmetric methods to
determinate the alkaline phosphatase (AF) activity and morphologic methods by means of
scanning. The cultivated cell showed a different morphology according to the degree of confluence
of the culture, being always positive the sucesive colourmetric determinations of AF
along the experiment. We consider the method of a great interest for orthopaedic investigations,
since it allows the study of the behaviour of these cells when they are subjet to different
types of external stimulus as well as meet they the new generations of biomaterials.
from the surface of bone trabeculae of explants obtained from femur and tibia metaphysis
in rabbits and human femoral head in patients undergoing orthopaedic surgery. Afther removing
the cells from the bone marrow of explants, they were placed over two mesh, 80 and
100 μmn, obtaining then a culture in monolayer which showed to be confluent after 20 days.
The isolated cell population showed an osteoblastic phenotype using colourmetric methods to
determinate the alkaline phosphatase (AF) activity and morphologic methods by means of
scanning. The cultivated cell showed a different morphology according to the degree of confluence
of the culture, being always positive the sucesive colourmetric determinations of AF
along the experiment. We consider the method of a great interest for orthopaedic investigations,
since it allows the study of the behaviour of these cells when they are subjet to different
types of external stimulus as well as meet they the new generations of biomaterials.
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