34-198
Injertos osteocondrales congelados en el conejo. Estudio experimental
Frozen osteocartilaginous allografts rabbits. Experimental study
A. CARRANZA-BENCANO, J.R. ARMAS-PADRÓN, E. GONZÁLEZ-FERNÁNDEZ, M.T. CAÑETE REINA CENTRO DE INSEMINACIÓN IN VITRO Y TRANSFERENCIA EMBRIONARIA DE SEVILLA. FACULTAD DE MEDICINA, UNIVERSIDAD DE SEVILLA.
Recepción:
15/09/2008
Aceptación:
15/09/2008
Publicación:
15/09/2008
Métricas
55
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Resumen (ES)
The purpose of this study was to analyze a protocol
for freezing whole cartilage and to study the viability of cartilage
after the implant in 60 knees of 45 New Zealand rabbits from a
genetically impure population. Osteocartilaginous implants were
removed, measuring 4 mm in diameter and 2-3 mm thickness,
including the articular cartilage and the subchondral bone, and
slow freezing up -196° using Planer Kryo-10, Series II, Controlled
Rate Freezers, following a controlled drop in temperature under
cryo-protection with Dimethylsulfoxide, propanediol and sacarose.
After thawing grafts were implanted in femoral condyles. The
implants evolves into an acellular amorphous tissue and after 2
months, period in wich the incorporation of the osseous compound
occurs, substitute fibrocartilaginous tissue develops. This
study shows that frozen articular cartilage does not remain viable
after implant.
for freezing whole cartilage and to study the viability of cartilage
after the implant in 60 knees of 45 New Zealand rabbits from a
genetically impure population. Osteocartilaginous implants were
removed, measuring 4 mm in diameter and 2-3 mm thickness,
including the articular cartilage and the subchondral bone, and
slow freezing up -196° using Planer Kryo-10, Series II, Controlled
Rate Freezers, following a controlled drop in temperature under
cryo-protection with Dimethylsulfoxide, propanediol and sacarose.
After thawing grafts were implanted in femoral condyles. The
implants evolves into an acellular amorphous tissue and after 2
months, period in wich the incorporation of the osseous compound
occurs, substitute fibrocartilaginous tissue develops. This
study shows that frozen articular cartilage does not remain viable
after implant.
Palabras clave (ES):
Injerto
Congelado
Aloinjerto
Conejo
Estudio
Experimental
Osteocondral
Cartílago
Óseo
Criopreservado
Resumen (EN)
The purpose of this study was to analyze a protocol
for freezing whole cartilage and to study the viability of cartilage
after the implant in 60 knees of 45 New Zealand rabbits from a
genetically impure population. Osteocartilaginous implants were
removed, measuring 4 mm in diameter and 2-3 mm thickness,
including the articular cartilage and the subchondral bone, and
slow freezing up -196° using Planer Kryo-10, Series II, Controlled
Rate Freezers, following a controlled drop in temperature under
cryo-protection with Dimethylsulfoxide, propanediol and sacarose.
After thawing grafts were implanted in femoral condyles. The
implants evolves into an acellular amorphous tissue and after 2
months, period in wich the incorporation of the osseous compound
occurs, substitute fibrocartilaginous tissue develops. This
study shows that frozen articular cartilage does not remain viable
after implant.
for freezing whole cartilage and to study the viability of cartilage
after the implant in 60 knees of 45 New Zealand rabbits from a
genetically impure population. Osteocartilaginous implants were
removed, measuring 4 mm in diameter and 2-3 mm thickness,
including the articular cartilage and the subchondral bone, and
slow freezing up -196° using Planer Kryo-10, Series II, Controlled
Rate Freezers, following a controlled drop in temperature under
cryo-protection with Dimethylsulfoxide, propanediol and sacarose.
After thawing grafts were implanted in femoral condyles. The
implants evolves into an acellular amorphous tissue and after 2
months, period in wich the incorporation of the osseous compound
occurs, substitute fibrocartilaginous tissue develops. This
study shows that frozen articular cartilage does not remain viable
after implant.
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