A densitometry and morphometric assessment by static image cytometry of 50 malignant bone tumors (32 osteosarcomas, 12 chondrosarcomas, and 6 malignant fibrous histiocytomas of bone) was performed in order to assess the validity of the technic in the pathobiological and prognostic evaluation of these neoplasms. A strong correlation between the morphometric features, cromatine distribution and histologic grade was found (Spearman; p < 0.05). These parameters are also statistically different for hight and low grade tumors in clinical staging. The stimation of DNA ploidy by optic densitometry is a significant prognostic factor in the multivariant analysis for the high grade tumors (COX; p < 0.001). Although Flow cytometry is a better procedure to evaluate DNA ploidy peaks, our study reveals static image cytometry as a useful technic for determine the cromatine model and objective assessment of the biological grade of bone neoplasms.

A densitometry and morphometric assessment by static image cytometry of 50 malignant bone tumors (32 osteosarcomas, 12 chondrosarcomas, and 6 malignant fibrous histiocytomas of bone) was performed in order to assess the validity of the technic in the pathobiological and prognostic evaluation of these neoplasms. A strong correlation between the morphometric features, cromatine distribution and histologic grade was found (Spearman; p < 0.05). These parameters are also statistically different for hight and low grade tumors in clinical staging. The stimation of DNA ploidy by optic densitometry is a significant prognostic factor in the multivariant analysis for the high grade tumors (COX; p < 0.001). Although Flow cytometry is a better procedure to evaluate DNA ploidy peaks, our study reveals static image cytometry as a useful technic for determine the cromatine model and objective assessment of the biological grade of bone neoplasms.